• Olschewski A
• Olschewski H
The RESPImmun Faculty
Leigh Marsh is a molecular biologist and geneticist who concentrates his research on the inflammatory processes underlying CLD and PH. He is particularly interested in the functional cross-talk between structural cells and recruited inflammatory cells, and how oxidative stress can influence these cellular interactions. He possesses a wide-ranging experience in animal models of CLD and relevant physiological read-outs. Within RESPImmun he collaborates with Julia Kargl, Gunther Marsche, Peter Valentin Tomazic and Ákos Heinemann on inflammatory cell crosstalk, with Eva Sturm on mechanisms of allergic asthma and Grażyna Kwapiszewska molecular signaling.
ProjectProject 6: Compartment-specific immunophenotyping in chronic obstructive pulmonary disease
Co-PI: Alexander Avian
BackgroundChronic obstructive pulmonary disease (COPD) is a highly heterologous disease with several know phenotypes; these can be distinguished by the pathology, degree of inflammation, or response to treatment. Similar to asthma, COPD is characterised by an upper airway obstruction and chronic inflammation; however, COPD patients also suffer from loss of the small conducting airways, and chronic destruction of the lung parenchyma resulting in emphysema. The presence of pulmonary hypertension (PH) in patients with COPD is associated with even worse survival. We have previously shown that the idiopathic form of PH (IPAH) presents with a distinct inflammatory cell landscape; however, there is only limited knowledge to which extent the inflammatory profile in COPD overlaps with IPAH, and contributes to vascular remodelling.
Hypothesis and objectivesHere, we hypothesize that inflammatory cell profiles in COPD are associated with vascular remodelling and can discriminate between different COPD phenotypes. We will determine the inflammatory cell landscape in COPD with and without associated PH and its relationship to vascular remodeling, functionally characterize regulated inflammatory cell populations and determine their implications for vascular remodeling.
MethodologyYears 1– 2: The student will make extensive use of flow cytometry and computational flow cytometry to characterize the inflammatory cell profile in COPD patients. Multivariate analysis including unbiased dimensionality reduction (
Input from collaborations within the RESPImmun programme